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Related Experiment Videos

Cloning grills: high throughput cloning for structural genomics.

Christopher Mehlin1, Erica E Boni, Jamie Andreyka

  • 1Structural Genomics of Pathogenic Protozoa (SGPP), Department of Biochemistry, University of Washington, Box 357350, Seattle, WA 98195, USA. cmehlin@u.washington.edu

Journal of Structural and Functional Genomics
|July 21, 2004
PubMed
Summary
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Cloning grills enable rapid, accurate plating of 96 cultures onto just eight agar plates. This method efficiently multiplies E. coli cultures for streamlined colony isolation.

Area of Science:

  • Microbiology
  • Biotechnology
  • Laboratory Techniques

Background:

  • Efficient microbial culture and isolation are crucial in biological research.
  • Traditional methods for streaking multiple cultures can be time-consuming and resource-intensive.
  • Standard agar plates limit the number of distinct cultures that can be plated simultaneously.

Purpose of the Study:

  • To introduce and evaluate the utility of cloning grills for enhanced microbial plating.
  • To demonstrate a method for increasing the throughput of bacterial cultures on agar plates.
  • To streamline the process of colony isolation for Escherichia coli (E. coli).

Main Methods:

  • Cloning grills, aluminum grids, are autoclaved and embedded in solidified agar within square petri dishes.

Related Experiment Videos

  • Each grill divides the plate into 12 lanes, each accommodating a single E. coli culture.
  • A 12-channel pipette is used to streak cultures, leveraging lane spacing compatible with 96-well plates.

    • Main Results:

    • Cloning grills enable the simultaneous streaking of 12 cultures per plate.
    • This technique allows for the plating of 96 cultures using only eight agar plates.
    • The method facilitates rapid and accurate colony isolation.

    Conclusions:

    • Cloning grills significantly enhance the efficiency of microbial plating and colony isolation.
    • The use of cloning grills optimizes resource utilization in microbiology labs.
    • This technique offers a scalable solution for high-throughput microbial culture experiments.