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Updated: Aug 23, 2026

Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models
Published on: April 26, 2024
Effect of traditional and alternative intracoronal bleaching agents on microhardness of human dentine
H K Chng1, A U J Yap, P Wattanapayungkul
1Faculty of Dentistry, Department of Restorative Dentistry, National University of Singapore, Singapore. rsdchk@nus.edu.sg
Abstract:
The purpose of this study was to compare the effect of traditional and alternative bleaching agents on microhardness of human dentine when used intracoronally. Thirty-six premolars were divided into six groups and bleaching agents were sealed into the pulp chambers as follows: group 1--distilled water (control), group 2--30% hydrogen peroxide solution, group 3--sodium perborate mixed with distilled water, group 4--sodium perborate mixed with 30% hydrogen peroxide solution, group 5--35% carbamide peroxide gel, group 6--35% hydrogen peroxide gel. Access cavities were sealed and the teeth were stored in distilled water at 37 degrees C. After 7 days, each tooth was sectioned at the cemento-enamel junction level and microhardness testing was carried out on dentine. The results showed that treatment with 35% hydrogen peroxide gel, 30% hydrogen peroxide solution and 35% carbamide peroxide gel reduced the microhardness of outer dentine to a small extent while treatment with sodium perborate mixed with water and sodium perborate mixed with 30% hydrogen peroxide solution did not significantly alter the microhardness of dentine.
