Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microglia phagocytose alloreactive CTL-damaged 9L gliosarcoma cells.

Nisha V Kulprathipanja1, Carol A Kruse

  • 1Department of Immunology, University of Colorado Health Sciences Center, 4200 E. 9th Avenue, B216, Denver, CO 80262, USA.

Journal of Neuroimmunology
|July 22, 2004
PubMed
Summary

Microglia in rat brains selectively engulf damaged glioma cells after T cell therapy. This immune response targets only tumor cells harmed by alloreactive cytotoxic T lymphocytes (aCTL), not healthy ones.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retroviral replicating vector-mediated gene therapy achieves long-term control of tumor recurrence and leads to durable anticancer immunity.

Neuro-oncology·2017
Same author

Temozolomide induces the expression of the glioma Big Potassium (gBK) ion channel, while inhibiting fascin-1 expression: possible targets for glioma therapy.

Expert opinion on therapeutic targets·2016
Same author

Rasmussen encephalitis tissue transfer program.

Epilepsia·2016
Same author

Efficacy of systemic adoptive transfer immunotherapy targeting NY-ESO-1 for glioblastoma.

Neuro-oncology·2015
Same author

Evidence for the involvement of gamma delta T cells in the immune response in Rasmussen encephalitis.

Journal of neuroinflammation·2015
Same author

Fascin-1 knock-down of human glioma cells reduces their microvilli/filopodia while improving their susceptibility to lymphocyte-mediated cytotoxicity.

American journal of translational research·2015

Area of Science:

  • Immunology
  • Neuro-oncology
  • Cellular Biology

Background:

  • Intracranial adoptive T cell therapy shows promise for brain tumors.
  • Understanding endogenous immune responses to cellular therapy is crucial.
  • Microglia play a key role in the brain's immune surveillance.

Purpose of the Study:

  • To investigate microglia's phagocytic activity towards alloreactive cytotoxic T lymphocyte (aCTL)-damaged and undamaged 9L gliosarcoma cells.
  • To assess microglia's role in the immune response following aCTL therapy in a rat glioma model.

Main Methods:

  • In vitro analysis of microglia phagocytosis of 9L gliosarcoma cells (damaged vs. undamaged) using cells from tumor-bearing rat brains.
  • In vivo assessment of microglia phagocytosis of CFSE-labeled aCTL-damaged 9L tumor cells within the rat brain post-intracranial infusion.

Related Experiment Videos

  • Analysis of cytokine presence in cell culture supernates.

    • Main Results:

    • Microglia isolated from tumor-bearing rat brains phagocytosed aCTL-damaged 9L cells in vitro (5.5+/-0.9%), but not undamaged cells.
    • Supernates from 9L or aCTL+9L co-cultures did not significantly alter microglia's phagocytic ability towards damaged glioma cells.
    • In vivo, 17.5+/-0.1% of microglia phagocytosed aCTL-damaged 9L tumor cells within the brain 3 days after infusion.

    Conclusions:

    • Microglia in the tumor microenvironment selectively phagocytose damaged glioma cells.
    • This selective phagocytosis occurs for both aCTL-damaged and undamaged glioma cells.
    • Microglia contribute to the clearance of damaged tumor cells following T cell-based immunotherapy.