Related Experiment Video
Updated: Aug 23, 2026
Isolation of Targeted Hypothalamic Neurons for Studies of Hormonal, Metabolic, and Electrical Regulation
Published on: August 4, 2023
Increased pro-thyrotropin-releasing hormone transcription in hypophysiotropic neurons of Lewis rats
Leonardo H Tonelli1, Chad A Gunsolly, Elena Belyavskaya
1Section on Neuroendocrine Immunology and Behavior, National Institute of Mental Health, DHHS, 36 Convent Drive MSC 4020, Building 36, Room 1A23, Bethesda, MD 20892-4020, USA.
Abstract:
The expression of pro-thyrotropin-releasing hormone (pro-TRH) mRNA was analyzed in the hypothalamus of inflammatory susceptible LEW/N and resistant F344/N rats at baseline and in adrenalectomized and lipopolysaccharide-treated animals. In saline-treated control animals, increased pro-TRH transcription was detected in LEW/N with respect to F344/N rats. This increased LEW/N pro-TRH expression was stable regardless of condition in contrast to F344/N increased pro-TRH transcription post-adrenalectomy and decreased pro-TRH after lipopolysaccharide administration. The LEW/N increase in pro-TRH mRNA was independent of changes in hormonal status suggesting alteration of the thyroid axis at the central level in this strain.
