Related Experiment Video

Updated: Aug 23, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Rapid and efficient polymerase chain reaction-based strategies for one-site and two-site mutagenesis

Zhilong Gong1, Hai Zhang, Stephan Gabos

  • 1Environmental Health Sciences, Department of Public Health Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2G3.

Analytical Biochemistry
|July 22, 2004
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Related Experiment Videos

Last Updated: Aug 23, 2026

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
07:04

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Published on: February 5, 2019

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
PCR01:32

PCR

Overview

Articles linked to this work by shared authors, journal, and citation graph.

Photoacoustic image-guided biomimetic nanoparticles targeting rheumatoid arthritis.

Proceedings of the National Academy of Sciences of the United States of America·2022

The Development of Intelligent Education and Teachers' Professional Informatization Led by an Intelligent Ubiquitous Network.

Computational intelligence and neuroscience·2022

Integrating teacher data literacy with TPACK: A self-report study based on a novel framework for teachers' professional development.

Frontiers in psychology·2022

Linearization of nonlinear frequency modulated continuous wave generation using model-based reinforcement learning.

Optics express·2022

Investigations on the preparation of ceramsite from petrochemical excess sludge.

Frontiers in chemistry·2022

Prevalence and Genetic Characteristics of Japanese Encephalitis Virus among Mosquitoes and Pigs in Hunan Province, China from 2019 to 2021.

Journal of microbiology and biotechnology·2022

A CRISPR/Cas9 multiplex nucleic acid sensing platform based on rolling circle amplification-mediated monomeric substrate generation.

Analytical biochemistry·2026

Identification of MDV and REV Using RPA-CRISPR/Cas12a Combined with Fluorescence and Lateral Flow Assays.

Analytical biochemistry·2026

Selective fluorescence turn-on detection of methylglyoxal using N-substituted NBD derivatives.

Analytical biochemistry·2026

Simultaneous GC-MS determination of unsubstituted, hydroxy-, amino-, and hydroxy-amino short-chain fatty acids together with amino acids in biological and food samples following derivatization.

Analytical biochemistry·2026

Validation of SARS-CoV-2 neutralization assay using VSV-based pseudovirus system.

Analytical biochemistry·2026

Development and validation of a mechanism-based Ba/F3-hGHR bioassay for the potency assessment of recombinant human growth hormone.

Analytical biochemistry·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us