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Related Experiment Videos

Xylanase production by Ganoderma lucidum on liquid and solid state fermentation.

K Malarvizhi1, K Murugesan, P T Kalaichelvan

  • 1Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai 600 025, India.

Indian Journal of Experimental Biology
|July 23, 2004
PubMed
Summary

Ganoderma lucidum efficiently produces xylanase enzyme under optimized conditions. Wheat bran proved to be the best substrate for solid-state fermentation, enhancing enzyme production.

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Area of Science:

  • Biotechnology and enzyme production
  • Mycology and fungal fermentation

Background:

  • Ganoderma lucidum is a white rot fungus with potential for enzyme production.
  • Xylanase is an important enzyme used in various industrial applications.

Purpose of the Study:

  • To optimize culture conditions for xylanase production by Ganoderma lucidum.
  • To evaluate different substrates for solid-state fermentation of xylanase.

Main Methods:

  • Shake and solid-state culture techniques were employed.
  • Optimization of pH, temperature, carbon, and nitrogen sources.
  • Agrowaste substrates (wheat bran, sugarcane bagasse, rice bran) were tested for solid-state fermentation.

Main Results:

  • Optimal pH range was 6.0-7.0, and temperature range was 30-35°C for growth and xylanase secretion.

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  • Xylan and peptone were identified as optimal carbon and nitrogen sources, respectively.
  • Wheat bran was the most effective substrate for xylanase production in solid-state fermentation compared to sugarcane bagasse and rice bran.

    • Conclusions:

    • Ganoderma lucidum can be effectively utilized for xylanase production.
    • Optimized conditions and substrate selection are crucial for maximizing enzyme yield.
    • Solid-state fermentation using wheat bran is a promising method for industrial xylanase production.