Related Experiment Video
Updated: Aug 23, 2026
Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography
Published on: January 17, 2020
Reduced photobleaching of chromophores close to a metal surface
Krasimir Vasilev1, Fernando D Stefani, Volker Jacobsen
1Max Planck Institut für Polymerforschung, Ackermannweg 10, 55128 Mainz, Germany.
Abstract:
The photobleaching of chromophores in front of a metal film is measured by recording the emitted fluorescence intensity from an ensemble of chromophores as a function of time. A strong dependence of the photostability on the distance from the metal surface is found. The experimental data are well described in a classical electromagnetic model with the additional assumption that photobleaching occurs at a constant rate from the excited state. The metal interface influences the photostability of the chromophores in two ways, first by altering the excitation rate by local enhancement of the electromagnetic field and second by altering the electromagnetic decay rate.
Related Concept Videos
00:56Extraction: Advanced Methods
01:24Microbial Corrosion
03:02Colors and Magnetism
When atoms or molecules absorb light at the proper frequency, their electrons are excited to higher-energy orbitals. For many main group atoms and molecules, the absorbed photons are in the ultraviolet range of the electromagnetic spectrum, which cannot be detected by the human eye. For coordination compounds, the energy difference between the d orbitals often allows photons in the visible range to be absorbed and emitted, which is seen as colors by the human eye.
02:49Corrosion
01:18Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a result, EDTA...