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[Study on the detection of duck hepatitis B virus with SYBR based quantitative real-time PCR]

Wen Zou1, Xu Yang, Li-Ping Yang

  • 1Liver Disease Research Center, the Second Xiangya Hospital, Central South University, Changsha 410011, China.

Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal of Hepatology
|July 23, 2004
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...