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Rescue of Recombinant Newcastle Disease Virus from cDNA
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Rescue of Recombinant Newcastle Disease Virus from cDNA

Published on: October 11, 2013

The P gene of Newcastle disease virus does not encode an accessory X protein

Ben Peeters1, Paul Verbruggen1, Frank Nelissen1

  • 1Division of Infectious Diseases, Animal Sciences Group, Wageningen University and Research Centre, PO Box 65, NL-8200 AB Lelystad, The Netherlands.

The Journal of General Virology
|July 23, 2004
PubMed

Insights

Newcastle disease virus (NDV) accessory X protein is not expressed from the P gene. Modifying the AUG codon reduced NDV virulence, impacting P and/or V protein function.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Paramyxoviruses often encode accessory proteins that modulate viral replication and host antiviral responses.
  • The P gene mRNA of Newcastle disease virus (NDV) was hypothesized to produce an accessory X protein via translation initiation at a specific AUG codon.

Purpose of the Study:

  • To investigate the expression of the putative accessory X protein from the NDV P gene.
  • To determine the functional significance of the AUG codon at position 120 in the NDV P gene.

Main Methods:

  • Utilized a specific monoclonal antibody to detect both P and X proteins in NDV-infected cells.
  • Generated recombinant NDV strains with altered AUG codons (to GCC or GUC) at position 120.
  • Assessed the virulence of recombinant NDV strains.

Main Results:

  • The accessory X protein was not detected in NDV-infected cells, refuting its expression.
  • Recombinant NDV strains with modified AUG codons remained viable.
  • These modified NDV strains exhibited reduced virulence compared to wild-type NDV.

Conclusions:

  • The proposed accessory X protein is not expressed by Newcastle disease virus.
  • The AUG codon at position 120 is critical, likely affecting the function of the P and/or V protein, thereby influencing NDV virulence.

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