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Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation
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Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

Published on: May 3, 2018

In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling

Amjad Javed1, Sayyed K Zaidi, Soraya E Gutierrez

  • 1Department of Cell Biology, University of Massachusetts Medical School, Worcester, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 23, 2004
PubMed
Abstract

No abstract available in PubMed .

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...