Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene transfer and expression in plants.

Argelia Lorence1, Robert Verpoorte

  • 1Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 23, 2004
PubMed
Summary

Modern plant breeding utilizes advanced molecular biology and genomics for genetic modification. Key technologies like Agrobacterium-mediated transformation and biolistics enable efficient DNA transfer for crop improvement.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A prebiotic-based biostimulant enhances growth parameters, photosynthetic efficiency, and grain yield in rice (<i>Oryza sativa</i> ssp. <i>japonica</i>).

Frontiers in plant science·2026
Same author

A technique for measuring non-structural carbohydrate reserves in flag leaves of paddy rice using Fourier transform infrared spectroscopy (FTIR).

Plant methods·2025
Same author

<i>Eleutherococcus senticosus</i> Fruit Extract Stimulates the Membrane Potential of the Trachea and Small Intestine in Rabbits.

Molecules (Basel, Switzerland)·2025
Same author

Natural Products Analysis Through Time: From Past Achievements to Future Prospects.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Extraction of Anthocyanins with Deep Eutectic Solvents and Chemical Evaluation by High-Performance Liquid Chromatography.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Natural variation in LONELY GUY-Like 1 regulates rice grain weight under warmer night conditions.

Plant physiology·2024

Area of Science:

  • Plant Molecular Biology
  • Genomics
  • Agricultural Biotechnology

Background:

  • Traditional agriculture relied on selective breeding and sexual gene transfer.
  • Recent advances in plant genomics offer unprecedented understanding and modification capabilities for plant genomes.

Purpose of the Study:

  • To provide an updated overview of key technologies for plant genetic material transfer.
  • To discuss variables, advantages, limitations, and applications of plant transformation methods.

Main Methods:

  • Agrobacterium-mediated transformation
  • Microparticle bombardment (biolistics)
  • In planta transformation systems

Main Results:

  • Detailed comparison of Agrobacterium-mediated transformation and biolistics, including efficiency factors.
  • Exploration of transient vs. stable transformation, marker-free approaches, and available genetic tools.
  • Analysis of transgene expression, gene silencing, and strategies to overcome expression challenges, such as using matrix-attachment regions (MARs).

Conclusions:

  • Agrobacterium-mediated transformation and biolistics are powerful tools for plant genetic modification.
  • Understanding transformation variables and genetic tools is crucial for efficient crop improvement and developing transgenic plants.

Related Experiment Videos