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Related Experiment Videos

Poly(dimethylsiloxane) microchip: microchannel with integrated open electrospray tip.

Malin Svedberg1, Monica Veszelei, Jan Axelsson

  • 1The Angström Laboratory, Division of Materials Science, Uppsala University, P.O. Box 534, SE-751 21 Uppsala, Sweden.

Lab on a Chip
|July 23, 2004
PubMed
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This study introduces a novel polymer microchip with an open tip designed for electrospray mass spectrometry (E-MS). The simplified, open-tip design ensures stable ion current, making it ideal for sensitive molecular analysis.

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Microfluidics

Background:

  • Electrospray mass spectrometry (E-MS) is a powerful analytical technique.
  • Microfluidic devices offer advantages in sample handling and analysis.
  • Existing microchip designs for E-MS can be complex to fabricate and assemble.

Purpose of the Study:

  • To present a novel polymer microchip with an open tip for electrospray mass spectrometry.
  • To simplify the fabrication and assembly of microfluidic chips for E-MS.
  • To demonstrate the performance of the open-tip microchip in terms of stability.

Main Methods:

  • Fabrication of Poly(dimethylsiloxane) (PDMS) microchips using nickel molds replicated from dry-etched silicon wafers.
  • Design of an open-tip configuration where a droplet forms at the microchannel end.

Related Experiment Videos

  • Characterization of the microchip's performance using a 1 microM myoglobin solution.

    • Main Results:

    • Successfully replicated microchips with microchannel widths around 50 micrometers.
    • Achieved a total ion current variation of 5% over 300 seconds for a myoglobin solution.
    • Demonstrated simplified assembly due to the open-tip design without a full cover.

    Conclusions:

    • The developed polymer microchip with an open tip is suitable for electrospray mass spectrometry.
    • The simplified design and fabrication process, utilizing nickel molds, are advantageous.
    • The microchip exhibits stable performance, making it a promising platform for E-MS applications and further prototyping.