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Isolation and expression analysis of the testis-specific gene, human OPPO1
Toshinobu Miyamoto1, Kazuo Sengoku, Hiroaki Hayashi
1Department of Obstetrics and Gynecology, Asahikawa Medical College, Asahikawa, Japan. toshim@asahikawa-med.ac.jp
Purpose:
To investigate human spermatogenesis, we isolated human testis-specific genes.
Methods:
Using mouse amino acid sequences, we found the region including homology in amino acid level in the human genome sequences. The primers encompassing introns were made and RT-PCR and RACE were carried out. The resultant PCR products were sequenced.
Results:
The full-length cDNA of human OPPO1 was isolated. It encodes 257 amino acid residues. The expression of the human OPPO1 was predominantly in the testis. On the other hand, partial cDNAs of ZNF8, GR194, GR219, GR093, GR046, GR163, and GR200 were expressed in the various tissues.
Conclusions:
Our data suggests that the human OPPO1 may play important roles in human spermatogenesis.