Identity of a membrane-bound vasoactive intestinal peptide-binding protein with calmodulin

D Stallwood¹, C H Brugger, B A Baggenstoss

¹Department of Anesthesia, University of Nebraska Medical Center, Omaha 68198-4455.

|September 25, 1992

Abstract:

A vasoactive intestinal peptide (VIP)-binding protein purified from guinea pig lung membranes (p18) was digested with trypsin, and the amino acid sequence of the peptide fragments was determined. The sequence of six tryptic fragments of p18 was identical with subsequences present in mammalian calmodulin. Authentic porcine brain calmodulin and p18 co-migrated on an sodium dodecyl sulfate-electrophoresis gel and displayed identical chromatographic behavior on a reverse phase high performance liquid chromatography column. The VIP-binding properties of p18 and calmodulin were indistinguishable. Both proteins displayed saturable and apparent high affinity binding of VIP, evidenced by potent inhibition of complexation with [Tyr10-125I]VIP by unlabeled VIP (IC50 = 6.0-8.1 nM). Rat growth hormone releasing factor and a C terminally extended form of VIP ([Leu17]VIP-GKR) also displayed potent inhibition of the binding (IC50 = 6.4 and 4 nM, respectively). These neuropeptides are potential modulators of calmodulin function.

Related Experiment Videos

Identity of a membrane-bound vasoactive intestinal peptide-binding protein with calmodulin

Related Concept Videos

Mercury intoxication disrupts tonic signaling in B cells, and may promote autoimmunity due to abnormal phosphorylation of STIM-1 and other autoimmunity risk associated phosphoproteins involved in BCR signaling.

Predictive capability of material screening by fast neutron activation analysis using laser-driven neutron sources.

Super-strong magnetic field-dominated ion beam dynamics in focusing plasma devices.

Direct observation of imploded core heating via fast electrons with super-penetration scheme.

Enhancing laser beam performance by interfering intense laser beamlets.

A multichannel gated neutron detector with reduced afterpulse for low-yield neutron measurements in intense hard X-ray backgrounds.

Identification of a conserved neutralizing epitope on the ASFV H240R protein by a nanobody that inhibits viral attachment.

G Protein Coupled Receptor Kinase 3 Regulates CCR7 Signaling, Trafficking, and T Cell Responses to CCL19 and CCL21.

Fascin in Dendritic Protrusions is Required for Synaptic Plasticity.

Nanobody-Based Characterization of Galectin-13 Reveals Immunomodulatory and Tumor-Promoting Functions.

Integrated glycoproteomics reveals site-specific N-glycosylation defects in phosphomannomutase 2 congenital disorder of glycosylation.

mRNA translational dynamics mediates totipotent-like reprogramming.

Hypothermia in the intensive care unit after surgery: a registry based retrospective cohort study.

Efficacy and safety of sibeprenlimab in IgA nephropathy: interim analysis of the China cohort in the phase 3 VISIONARY trial.

Network pharmacology and molecular docking-based prediction of the multi-target mechanisms by which icariin intervenes in perimenopausal depressive-like symptoms.

Pharmacokinetics of IL-23p19 Inhibitors: A Systematic Review Across Immune-Mediated Inflammatory Diseases.

IgG Glycosylation Patterns May Identify Distinct Clinical Trajectories in Fibromyalgia Patients: A Longitudinal Pilot Study.

Detection of Intratumoral Immune Cell Antigens in an Immunocompetent Glioblastoma Stem-like Cell Model Using Substrate-Based Immunohistochemistry.

Related Experiment Videos

Identity of a membrane-bound vasoactive intestinal peptide-binding protein with calmodulin

Abstract:

Related Concept Videos

Related Articles