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YAPI, a new Yersinia pseudotuberculosis pathogenicity island.

François Collyn1, Alain Billault, Chantal Mullet

  • 1E0364 INSERM, Faculté de Médecine Henri Warembourg, Université de Lille II, and Institut de Biologie de Lille, F-59021 Lille, France.

Infection and Immunity
|July 24, 2004
PubMed
Summary

Pathogenicity islands (PAIs) are mobile DNA elements carrying virulence genes. A 98-kb PAI, YAPI, was identified in Yersinia pseudotuberculosis, containing adhesion genes and mobile elements, and capable of excision.

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Area of Science:

  • Microbiology
  • Genomics
  • Bacterial Pathogenesis

Background:

  • Pathogenicity islands (PAIs) are crucial for bacterial virulence, often integrating into tRNA genes.
  • Yersinia pseudotuberculosis harbors virulence factors, but the full extent of its mobile genetic elements is not fully understood.

Purpose of the Study:

  • To characterize a novel pathogenicity island in Yersinia pseudotuberculosis.
  • To investigate the genetic content, acquisition, and functional implications of this PAI.

Main Methods:

  • Genomic analysis of a 98-kb segment in Yersinia pseudotuberculosis 32777.
  • Identification of open reading frames, gene clusters, and mobile genetic elements.
  • Assessment of PAI excision and its impact on bacterial virulence.

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Main Results:

  • A 98-kb pathogenicity island, YAPI, was identified, integrating into a phenylalanine tRNA gene.
  • YAPI contains 95 open reading frames, including a type IV pilus operon, metabolic genes, and a restriction-modification system.
  • YAPI exhibits mosaic G+C content, indicating horizontal gene transfer, and can excise precisely from the chromosome.

Conclusions:

  • YAPI is a horizontally acquired pathogenicity island contributing to Yersinia pseudotuberculosis virulence.
  • The prevalence and integration sites of YAPI vary among strains.
  • YAPI is absent in Yersinia pestis but present in Yersinia enterocolitica, suggesting species-specific distribution.