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Updated: Aug 23, 2026

Chromatin Accessibility Profiling in Whole Caenorhabditis elegans L4 Larvae
Published on: April 17, 2026
Restriction endonuclease accessibility as a determinant of altered chromatin structure
William M Hempel1, Pierre Ferrier
1Department of Research Technologies, Pfizer Global Research and Development, Fresnes Cedex, France.
Abstract:
Active genes in eukaryotic genomes are typically found in open, nuclease-sensitive regions of chromatin. This chapter presents an overview of the techniques used to assay restriction endonuclease cleavage of chromosomal DNA as an approach to assess general accessibility at a genomic region of interest. We describe protocols to (1) prepare nuclei templates, (2) treat chromosomal DNA with a restriction enzyme(s), and (3) visualize and quantify chromosomal cleavage(s), with an emphasis on ligation-mediated (LM) PCR techniques.
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