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Related Concept Videos

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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Related Experiment Video

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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Complications of sinusitis.

M Wagenmann1, R M Naclerio

  • 1Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Md.

The Journal of Allergy and Clinical Immunology
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

Sinusitis complications, though rare, can be life-threatening, particularly orbital and intracranial issues. Prompt diagnosis and specialist treatment, including sinus drainage, are crucial for preventing severe outcomes and vision loss.

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Area of Science:

  • Otolaryngology
  • Neurology
  • Ophthalmology

Background:

  • Sinusitis complications, while infrequent, pose significant life-threatening risks.
  • These complications can manifest locally, orbitally, or intracranially, or as combined issues.

Purpose of the Study:

  • To highlight the critical nature of sinusitis complications.
  • To emphasize the importance of early recognition and prompt management.

Main Methods:

  • Review of potential complications of sinusitis.
  • Emphasis on diagnostic modalities like computed tomographic (CT) and magnetic resonance imaging (MRI) scans.
  • Discussion of treatment strategies including specialist intervention and sinus drainage.

Main Results:

  • Orbital complications are the most common, with acute ethmoiditis in children being a particular risk factor.
  • Intracranial complications may present with subtle symptoms, necessitating advanced imaging for diagnosis.
  • Immediate and aggressive therapy is vital to prevent permanent vision loss.

Conclusions:

  • Orbital and intracranial complications of sinusitis are medical emergencies requiring specialist care.
  • Early diagnosis through advanced imaging and timely sinus infection drainage are paramount.
  • Physicians must maintain a high index of suspicion for sinusitis complications due to their potential severity.