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Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
AMID is a p53-inducible gene downregulated in tumors
Min Wu1, Liang-Guo Xu, Tian Su
1Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
Abstract:
AMID, also called PRG3, is an AIF-homologous and mitochondria-associated protein that has been implicated in caspase-independent apoptosis. In this report, we demonstrated that human AMID gene promoter was activated by p53 in reporter gene assays. Chromatin immunoprecipitation experiments indicated that p53 could bind to human AMID promoter. Deletion mutagenesis indicated that human AMID promoter contains two p53-responsive elements. Furthermore, expression array analysis indicated that human AMID mRNA expression was downregulated in a majority of human tumors. Our findings point to the possibility that AMID is a p53-downstream gene involved in tumorigenesis.
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