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Mouse-centric comparative transcriptomics of protein coding and non-coding RNAs
Masanori Suzuki1, Yoshihide Hayashizaki
1Laboratory for Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, Kanagawa, Japan.
Summary
The largest mouse transcriptome dataset reveals extensive alternative splicing and numerous non-coding RNAs (ncRNAs). These ncRNAs play crucial roles in gene regulation and genome evolution.
Area of Science:
- Transcriptomics
- Genomics
- RNA Biology
Background:
- The largest mouse full-length cDNA transcriptome dataset provides a valuable reference for comparative transcriptomics.
- Mouse cDNA numbers significantly surpass predicted gene counts due to alternative splicing and non-coding RNAs (ncRNAs).
Purpose of the Study:
- To review transcriptome data across various organisms, using mouse data as a reference.
- To elucidate the regulatory mechanisms and physiological significance of abundant ncRNAs.
Main Methods:
- Comparative analysis of transcriptome data.
- Focus on mouse full-length cDNA clones and ncRNA identification.
Main Results:
- Extensive alternative splicing and a high abundance of ncRNAs are key features of mammalian transcriptomes.
- ncRNAs are integral to gene expression regulation and biological processes.
- Sense-antisense ncRNA pairs are prevalent and may drive genome evolution.
Conclusions:
- ncRNAs are major transcriptome components with significant regulatory functions.
- Understanding ncRNAs is crucial for comprehending gene expression, biological processes, and genome evolution.