Related Experiment Video

Updated: Dec 30, 2025

Quantitative Analysis of Mitochondria-Associated Endoplasmic Reticulum Membrane (MAM) Stabilization in a Neural Model of Alzheimer's Disease (AD)
06:41

Quantitative Analysis of Mitochondria-Associated Endoplasmic Reticulum Membrane (MAM) Stabilization in a Neural Model of Alzheimer's Disease (AD)

Published on: January 10, 2025

1.1K

MT1-MMP: an enzyme with multidimensional regulation

Yoshifumi Itoh1, Motoharu Seiki

  • 1Department of Matrix Biology, Kennedy Institute of Rheumatology Division, Imperial College London, Hammersmith, London W6 8LH, UK.

Trends in Biochemical Sciences
|July 28, 2004
PubMed

Insights

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is vital for cell functions but also disease. Its activity is precisely controlled through O-glycosylation, CD44 interactions, and cellular trafficking.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Membrane-type 1 matrix metalloproteinase (MT1-MMP) plays a dual role in physiological processes and disease pathogenesis.
  • MT1-MMP's activity as both a proteinase and a membrane protein requires precise regulation.
  • Understanding MT1-MMP regulation is crucial for targeting its involvement in various cellular functions.

Purpose of the Study:

  • To elucidate the multifaceted regulatory mechanisms governing MT1-MMP activity.
  • To highlight the significance of post-translational modifications and protein interactions in controlling MT1-MMP function.
  • To emphasize the spatial and temporal control of MT1-MMP on the cell surface.

Main Methods:

  • Review of recent studies on MT1-MMP regulation.
  • Analysis of O-glycosylation's impact on MT1-MMP.
  • Investigation of CD44 interaction and cellular internalization/recycling pathways.

Main Results:

  • MT1-MMP function is modulated by O-glycosylation.
  • Interaction with CD44 influences MT1-MMP activity.
  • Internalization and recycling are key mechanisms for MT1-MMP regulation.

Conclusions:

  • MT1-MMP regulation involves complex, multidimensional mechanisms.
  • O-glycosylation, CD44 interaction, and trafficking ensure precise spatial and temporal control of MT1-MMP.
  • These regulatory processes are essential for MT1-MMP's proper cell surface functioning.

More Related Videos

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch07:51

A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

Published on: December 10, 2020

6.0K
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition09:05

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

Published on: August 20, 2016

8.3K

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult...
3.1K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Regulation of Metabolism01:19

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.3K
The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
11.6K
The Extracellular Matrix01:42

The Extracellular Matrix

Overview
87.9K