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Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
Published on: May 13, 2016
Mitochondrial gene introgression between spined loaches via hybridogenesis
Kenji Saitoh1, Ik-Soo Kim, Eun-Hee Lee
1Tohoku National Fisheries Research Institute, Shinharam, Shiogama 985-0001, Japan. ksaitoh@affrc.go.jp
Abstract:
This report deals with an unusual mode of mitochondrial gene introgression between Cobitis hankugensis (C. sinensis) and C. longicorpus which is mediated by a unisexual hybridogenetic system of diploid-triploid C. hankugensis-longicorpus complex. Mitochondrial DNA sequences of 3329-3330bp encompassing from upstream ND6 to 12S rDNA indicated that mitochondrial genomes from the diploid hybrids, triploid hybrids, and their parental species are almost identical. Because triploid hybrids produce haploid ova with C. hankugensis chromosome set, normal diploid C. hankugensis regenerates upon insemination with C. hankugensis sperm. If the hybrid carries C. longicorpus mitochondrial genome, the regenerated C. hankugensis is a nucleo-cytoplasmic hybrid, thus accomplishing the unusual mode of mitochondrial gene introgression.
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