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Related Experiment Videos

Oil-soluble dyes incorporated in meridic diet of Diatraea grandiosella (Lepidoptera: Crambidae) as markers for adult

Jawwad A Qureshi1, Lawrent L Buschman, James E Throne

  • 1Department of Entomology, Kansas State University, Manhattan, KS 66506, USA.

Journal of Economic Entomology
|July 29, 2004
PubMed
Summary
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Two oil-soluble dyes, Sudan Red 7B and Sudan Blue 670, effectively marked southwestern corn borers for dispersal studies. The dyes proved reliable for marking all life stages and were suitable for mark-release-recapture experiments.

Area of Science:

  • Entomology
  • Pest Management

Background:

  • Mark-release-recapture studies are crucial for understanding insect dispersal.
  • Effective marking methods are essential for distinguishing released insects from wild populations.

Purpose of the Study:

  • To evaluate the efficacy of two oil-soluble dyes, Sudan Red 7B and Sudan Blue 670, for marking the southwestern corn borer, Diatraea grandiosella.
  • To assess the impact of these dyes on the survival, development, and reproductive success of the insect.

Main Methods:

  • Southwestern corn borers were reared on diets containing either Sudan Red 7B or Sudan Blue 670, with an untreated control group.
  • Evaluated parameters included survival, diet consumption, weight, development time, fecundity, longevity, and adult mating success.
  • Marker retention and visibility across different life stages were assessed.

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Main Results:

  • Both dyes reliably marked eggs, larvae, pupae, and adults, with color retention throughout the adult lifespan.
  • Minor effects on insect development and consumption were observed, but did not prevent successful mating.
  • First-generation progeny survived but tended to lose the marker when fed untreated diet.

Conclusions:

  • Sudan Red 7B and Sudan Blue 670 are suitable for marking southwestern corn borers for mark-release-recapture dispersal studies.
  • The dyes are also applicable for short-term studies involving marked larvae and oviposition behavior.