Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Superoxide dismutase: enhanced small intestinal preservation.

S C Sun1, S M Greenstein, R S Schechner

  • 1Department of Surgery, Montefiore Medical Center, Bronx, New York 10467.

The Journal of Surgical Research
|June 1, 1992
PubMed
Summary

Adding superoxide dismutase (SOD) to University of Wisconsin (UW) solution significantly improved small intestine preservation. SOD-modified UW solution enhanced mucosal absorption and survival rates in rat studies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Conservative observational management with selective delayed repair for large abdominal aortic aneurysms in high risk patients.

The Journal of cardiovascular surgery·2003
Same author

Endovascular approaches to ruptured infrarenal aorto-iliac aneurysms.

The Journal of cardiovascular surgery·2002
Same author

Overt colon ischemia after endovascular aneurysm repair: the importance of microembolization as an etiology.

Journal of vascular surgery·2001
Same author

Eversion technique increases the risk for post-carotid endarterectomy hypertension.

Journal of vascular surgery·2001
Same author

Limited role for IVUS in the endovascular repair of aortoiliac aneurysms.

The Journal of cardiovascular surgery·2001
Same author

Endoscopically assisted in situ lower extremity bypass graft: a preliminary report of a new minimally invasive technique.

Journal of vascular surgery·2001

Area of Science:

  • Organ preservation
  • Free radical scavenging
  • Gastrointestinal surgery

Background:

  • Oxygen free radical injury is a significant concern in small intestine preservation.
  • Existing preservation solutions may not fully mitigate this oxidative damage.

Purpose of the Study:

  • To evaluate the efficacy of superoxide dismutase (SOD), a free radical scavenger, when combined with University of Wisconsin (UW) solution for small intestine preservation.
  • To assess the impact of SOD-modified UW solution on acute and chronic small intestine viability.

Main Methods:

  • Segments of rat small intestine were flushed and stored for 18 hours at 4°C in Collins, UW, or SOD-modified UW (8000 U/ml) solutions.
  • Acute study involved 2-hour reperfusion with SOD administration during reperfusion.

Related Experiment Videos

  • Chronic study involved isograft transplantation with pre-reperfusion SOD administration to recipients.

    • Main Results:

    • The UW/SOD group exhibited superior recovery of mucosal absorption (256 ± 39 vs. 202 ± 21 in Collins, P<0.01).
    • UW/SOD group showed the least percentage weight gain (19 ± 3% vs. 25 ± 5% in UW, P<0.01).
    • The UW/SOD group demonstrated the highest 17-day survival rate (9/12 vs. 2/9 in UW, P<0.025).

    Conclusions:

    • Superoxide dismutase (SOD) significantly enhances the preservation of small intestine when added to University of Wisconsin (UW) solution.
    • SOD-modified UW solution improves functional recovery and long-term graft survival.
    • This approach offers a promising strategy for reducing oxidative injury in intestinal preservation.