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Updated: Aug 23, 2026

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Published on: February 13, 2012
Store-operated calcium entry: a tough nut to CRAC
Reinhold Penner1, Andrea Fleig
1Laboratory of Cell and Molecular Signaling, Center for Biomedical Research at The Queen's Medical Center and John A. Burns School of Medicine at the University of Hawaii, Honolulu, HI 96813, USA. rpenner@hawaii.edu
Abstract:
Store-operated or capacitative Ca2+ entry is a prominent feature of many electrically nonexcitable cell types. It is due to Ca2+ ion permeation pathways in the plasma membrane that are activated after receptor-mediated Ca2+ release from intracellular stores. Despite hundreds of publications on the topic of store-operated Ca2+ entry and intense efforts by many dedicated laboratories, neither the molecular nature of the ion permeation pathway nor its activation mechanism is known. Here we review the progress made on the characterization of store-operated currents and the challenges encountered in identifying the molecular components of store-operated Ca2+ entry.
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