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Updated: Aug 23, 2026
The Use of Reverse Phase Protein Arrays (RPPA) to Explore Protein Expression Variation within Individual Renal Cell Cancers
Published on: January 22, 2013
Effects of resveratrol on gene expression in renal cell carcinoma
Ting Shi1, Louis S Liou, Provash Sadhukhan
1Department of Cancer Biology, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
Abstract:
Studies have shown that Resveratrol (RE) can inhibit cancer initiation, promotion, and progression. However the gene expression profile in renal cell carcinoma (RCC) in response to RE treatment has never been reported. To understand the potential anticancer effect of RE on RCC at molecular level, we profiled and analyzed the expression of 2059 cancer-related genes in a RCC cell line RCC54 treated with RE. Biological functions of 633 genes were annotated based on biological process ontology and clustered into functional categories. Twenty-nine highly differentially expressed genes in RE treated RCC54 were identified and the potential implications of some gene expression alterations in RCC carcinogenesis were identified. RE was also shown to inhibit cell growth and induce cell death of RCC cells. The expression alterations of selected genes were validated using reverse transcription polymerase chain reaction. In addition, the gene expression profiles under different RE treatments were analyzed and visualized using singular value decomposition. The findings from this study support the hypothesis that RE induces differential expression of genes that are directly or indirectly related to the inhibition of RCC cell growth and induction of RCC cell death. In addition, it is apparent that the gene expression alterations due to RE treatment depend strongly on RE concentration. This study provides a general understanding of the overall genetic response of RCC54 to RE treatment and yields insights into the understanding of the cancer preventive mechanism of RE in RCC.
Insights
Resveratrol (RE) shows potential in treating renal cell carcinoma (RCC) by altering gene expression and inhibiting cancer cell growth. This study reveals RE
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Resveratrol (RE) is known for its potential anticancer properties.
- The molecular mechanisms of RE in renal cell carcinoma (RCC) are not fully understood.
- Gene expression profiling provides insights into cellular responses to treatments.
Purpose of the Study:
- To investigate the gene expression profile of RCC cells treated with RE.
- To identify differentially expressed genes and their functions in RCC.
- To understand the molecular basis of RE's anticancer effects on RCC.
Main Methods:
- Profiling of 2059 cancer-related genes in RCC54 cells treated with RE.
- Functional annotation and clustering of 633 differentially expressed genes.
- Validation of gene expression using reverse transcription polymerase chain reaction (RT-PCR).
Main Results:
- Identified 29 highly differentially expressed genes in RE-treated RCC54 cells.
- RE inhibited RCC cell growth and induced cell death.
- Gene expression alterations were dependent on RE concentration.
Conclusions:
- RE induces differential gene expression related to RCC cell growth inhibition and cell death.
- This study provides insights into the cancer-preventive mechanisms of RE in RCC.
- RE's effects on RCC are concentration-dependent.
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