Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Islet-1 expression in the developing chicken inner ear.

Huawei Li1, Hong Liu, Cyrille Sage

  • 1Department of Otolaryngology and Program in Neuroscience, Harvard Medical School, and Eaton Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, Massachusetts 02114, USA.

The Journal of Comparative Neurology
|July 29, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rescue of Hearing by Gene Delivery to Inner-Ear Hair Cells Using Exosome-Associated AAV.

Molecular therapy : the journal of the American Society of Gene Therapy·2017
Same author

Adult-born dentate neurons are recruited in both spatial memory encoding and retrieval.

Hippocampus·2015
Same author

Overlapping and distinct pRb pathways in the mammalian auditory and vestibular organs.

Cell cycle (Georgetown, Tex.)·2011
Same author

Diverse expression patterns of LIM-homeodomain transcription factors (LIM-HDs) in mammalian inner ear development.

Developmental dynamics : an official publication of the American Association of Anatomists·2008
Same author

Biodiversity and biogeography of phages in modern stromatolites and thrombolites.

Nature·2008
Same author

Simultaneous detection of three fish rhabdoviruses using multiplex real-time quantitative RT-PCR assay.

Journal of virological methods·2008
Same journal

Inner Ear Anatomy of Caenagnathidae (Theropoda: Oviraptorosauria) Emphasizes Mosaic Evolution of the Avian Neurosensory System.

The Journal of comparative neurology·2026
Same journal

Inhibitory Neurons in Human Anterior Entorhinal Cortex and Some Comparisons With the Rhesus Monkey.

The Journal of comparative neurology·2026
Same journal

In Situ Hybridization Chain Reaction and Immunohistochemical Labeling of the Octopamine Production Pathway in the Central Nervous System of Lymnaea stagnalis.

The Journal of comparative neurology·2026
Same journal

Innervation Pattern of Inhibitory Projection Neurons in the Bird Sound Localization Circuit.

The Journal of comparative neurology·2026
Same journal

Mu Opioid Receptor mRNA and Protein Localization Across the Rat and Mouse Habenula.

The Journal of comparative neurology·2026
Same journal

Proline-Rich Transmembrane Protein 2 Is Variably Expressed Across Excitatory and Inhibitory Neurons in Mouse Motor Circuits.

The Journal of comparative neurology·2026
See all related articles

The LIM/homeodomain protein islet-1 is crucial for inner ear development, guiding the differentiation of auditory and vestibular neurons and sensory cells. Its expression patterns reveal key roles in cell fate specification within the developing inner ear.

Area of Science:

  • Developmental biology
  • Neuroscience
  • Genetics

Background:

  • Inner ear cell types develop from the otic placode, forming the otocyst.
  • Cochleovestibular neurons arise from the ventral otocyst.
  • The role of specific transcription factors in inner ear development is not fully understood.

Purpose of the Study:

  • To investigate the expression patterns and function of the LIM/homeodomain protein islet-1 in inner ear development.
  • To determine the role of islet-1 in the differentiation of inner ear neurons and sensory epithelia cells.

Main Methods:

  • Immunohistochemistry to detect islet-1 expression in developing inner ear tissues.
  • Analysis of gene expression patterns during otic development.
  • Correlation of islet-1 expression with BMP4 and Pax-2 expression domains.

Related Experiment Videos

Main Results:

  • Islet-1 is expressed in the ventral otic placode, pit, and otocyst, marking the origin of auditory and vestibular neurons.
  • Islet-1 expression is maintained in mature inner ear neurons and up-regulated in developing sensory epithelia.
  • Islet-1 up-regulation coincides with BMP4 expression and Pax-2 down-regulation in sensory cell precursors.

Conclusions:

  • Islet-1 plays a significant role in differentiating inner ear neurons and sensory epithelia.
  • Islet-1 may be involved in specifying cellular subtypes within the inner ear.
  • Further research into LIM/homeodomain proteins could elucidate inner ear development mechanisms.