Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Correction for liquid junction potentials in patch clamp experiments.

E Neher

    Methods in Enzymology
    |January 1, 1992
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus.

    Molecular psychiatry·2016
    Same author

    Venus Flytrap HKT1-Type Channel Provides for Prey Sodium Uptake into Carnivorous Plant Without Conflicting with Electrical Excitability.

    Molecular plant·2015
    Same author

    Introduction: regulated exocytosis.

    Cell calcium·2012
    Same author

    Kinetics of both synchronous and asynchronous quantal release during trains of action potential-evoked EPSCs at the rat calyx of Held.

    The Journal of physiology·2007
    Same author

    Optimizing imaging parameters for the separation of multiple labels in a fluorescence image.

    Journal of microscopy·2003
    Same author

    Calcium signals and synaptic short-term plasticity in the central nervous system.

    Anales de la Real Academia Nacional de Medicina·2002
    Same journal

    Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

    Methods in enzymology·2026
    Same journal

    A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

    Methods in enzymology·2026
    Same journal

    Evolutionary genetic approaches to analyze mucins.

    Methods in enzymology·2026
    Same journal

    Ex vivo imaging and enzymatic analysis of intestinal mucus.

    Methods in enzymology·2026
    Same journal

    Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

    Methods in enzymology·2026
    Same journal

    Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

    Methods in enzymology·2026
    See all related articles

    Accurate membrane potential measurements in patch clamp experiments require corrections for liquid junction potentials. These corrections are essential when pipette and bath solutions differ or when the bath solution is changed during the experiment.

    Area of Science:

    • Electrophysiology
    • Biophysics

    Background:

    • Patch clamp electrophysiology is a key technique for studying ion channel function.
    • Accurate measurement of membrane potentials is crucial for interpreting electrophysiological data.
    • Liquid junction potentials can introduce significant errors in measured membrane potentials.

    Purpose of the Study:

    • To describe necessary corrections for liquid junction potentials in patch clamp experiments.
    • To provide guidance on minimizing and correcting for junction potentials during solution changes.
    • To highlight the thermodynamic limitations in precisely defining liquid junction potentials.

    Main Methods:

    • Application of equations (Eqs. 1-3) for initial junction potential correction when pipette and bath solutions differ.
    • Implementation of specific protocols, including the use of a 3 M KCl salt bridge, to manage junction potentials during bath solution changes.

    Related Experiment Videos

  • Utilizing approximate correction equations (Eqs. 6-8) for localized solution changes or recessed salt bridges.

    • Main Results:

    • Identified two main scenarios requiring junction potential corrections: differing initial solutions and during-experiment bath solution changes.
    • Demonstrated that a 3 M KCl salt bridge with an abrupt tip minimizes but does not eliminate potential issues like contamination.
    • Provided approximate equations for calculating corrections in more complex experimental setups.

    Conclusions:

    • Precise measurement of membrane potentials in patch clamp requires careful consideration and correction of liquid junction potentials.
    • The choice of reference electrode setup and solution exchange strategy significantly impacts the accuracy of potential measurements.
    • Acknowledged that the provided equations offer approximate corrections due to the inherent thermodynamic ambiguity of liquid junction potentials.