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Updated: Jul 31, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Enzymatic and hybridization properties of oligonucleotide analogs containing novel phosphoramidate internucleotide
Ahmed M Awad1, Michal Sobkowski, Hartmut Seliger
1Sektion Chemische Funktionen in Biosystemen, University of Ulm, Ulm, Germany.
Abstract:
In line with the paradigm, that antisense oligonucleotides should contain minimal structural modifications, in order to minimize the risk of toxicity and antigenicity, we describe here the preparation and the properties of oligonucleotides modified to contain, in addition to phosphodiester bonds, a small number of phosphoramidate internucleotide linkages substituted with aminoethoxyethyl groups in order to convey protection against exo- and endonucleases. Prolonged stability was, in fact, found in model experiments with respective enzymes, as well as in studies done in human blood serum. Regardless of number and position of phosphoramidate linkages, the modified oligonucleotides showed only a slight decrease of Tm in hybridization studies with complementary oligonucleotides.
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