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Updated: Aug 23, 2026

Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
Published on: June 13, 2021
[Isolation of cell membranes from Saccharomyces cerevisiae for evaluation of the protein composition]
D A Aliverdieva1, A G Malygin, L S Lagutina
1Pricaspian Institute for Bioresource, Dagestan Research Center, Russian Academy of Sciences, Makhachkala, 367025 Russia.
Abstract:
We describe a simple method for the isolation of membrane fractions from Saccharomyces cerevisiae yeasts, containing the complex of plasma membranes and cell walls. The method is based on cell disruption on an INBI flow disintegrator. This device spares subcellular structures, which simplifies the isolation of cell membranes. The membrane fraction obtained by this method was suitable for studies of protein composition of these structures by means of two-dimensional electrophoresis.
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