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Updated: Aug 23, 2026

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
07:26

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis

Published on: October 4, 2018

Pathogenicity and molecular analysis of an infectious bursal disease virus isolated from Malaysian village chickens

D Y Tan1, M Hair-Bejo, A R Omar

  • 1Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Avian Diseases
|July 31, 2004
PubMed

Insights

Infectious Bursal Disease Virus (IBDV) in village chickens poses a significant threat. This study characterized a very virulent IBDV strain, highlighting its role in disease spread and the need for control measures.

Area of Science:

  • Veterinary Virology
  • Avian Pathology
  • Molecular Epidemiology

Background:

  • The epidemiology of Infectious Bursal Disease Virus (IBDV) in non-commercial avian species remains poorly understood.
  • Village chickens, descendants of jungle fowl, are common in backyards and may harbor avian pathogens.

Purpose of the Study:

  • To characterize an Infectious Bursal Disease Virus (IBDV) isolate from a Malaysian village chicken outbreak.
  • To determine the pathogenicity and molecular features of the IBDV isolate.
  • To assess the potential role of village chickens in IBDV epidemiology.

Main Methods:

  • In vivo pathogenicity studies in village and specific-pathogen-free chickens.
  • Molecular characterization of the IBDV isolate, including VP2 gene analysis.
  • Phylogenetic analysis using distance, maximum parsimony, and maximum likelihood methods.

Main Results:

  • The IBDV isolate (94268) was identified as a very virulent IBDV (vvIBDV) strain, causing high mortality (>88%) in village chickens and significant mortality (>66%) in SPF chickens.
  • The isolate possessed all molecular markers associated with vvIBDV in its VP2 gene.
  • Phylogenetic analysis confirmed the isolate's close relationship to other vvIBDV strains, particularly Malaysian isolates.

Conclusions:

  • Village chickens can harbor and spread very virulent IBDV strains.
  • The genetic similarity of the village chicken IBDV isolate to those in commercial chickens suggests cross-farm transmission.
  • Village chickens are a critical, yet often overlooked, reservoir in IBDV epidemiology and disease control efforts.

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