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Electron capture dissociation in a radio frequency ion trap
Takashi Baba1, Yuichiro Hashimoto, Hideki Hasegawa
1Central Research Laboratory, Hitachi, Ltd., Kokubunji, Tokyo 185-8601, Japan. baba@harl.hitachi.co.jp
Analytical Chemistry
|July 31, 2004
Summary
Researchers demonstrate electron capture dissociation (ECD) in a radio frequency (rf) ion trap for peptide analysis. This breakthrough enables high-throughput, low-cost molecular structure determination, complementing existing methods.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Physical Chemistry
Background:
- Electron capture dissociation (ECD) is a powerful technique for peptide and protein analysis.
- Implementing ECD in radio frequency (rf) ion traps has been challenging due to rf field interference.
Purpose of the Study:
- To report the first evidence of successful electron capture dissociation (ECD) in a radio frequency (rf) ion trap.
- To investigate a novel electron injection scheme for ECD in rf traps.
Main Methods:
- Peptide ions ([substance P]2+) were trapped in a linear rf ion trap.
- Electrons were injected along the trap's central axis where the rf field component is zero.
- A 50 mT magnetic field was applied, and electron energy was kept below 1 eV.
Main Results:
- Electron capture dissociation (ECD) was successfully achieved in the rf ion trap.
- An ECD efficiency of approximately 4% was observed with 0.2 microA electron current for 80 ms.
- The electron injection scheme prevented electron heating by the rf field.
Conclusions:
- Electron capture dissociation (ECD) is feasible in radio frequency (rf) ion traps.
- This method offers potential for high-throughput and low-cost molecular structure information.
- ECD in rf traps provides complementary data to collision-induced dissociation.