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Updated: Aug 23, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System
Published on: February 23, 2018
Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases
1Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, Hawaii 96822, USA. alvarez@hawaii.edu
Abstract:
Disease diagnosis is based on a number of factors, including laboratory tests for pathogen identification. Rapid development of genomic techniques for characterization of bacteria over the past decade has greatly simplified and improved pathogen detection and identification, but DNA-based methods have not yet entirely replaced traditional culture and phenotypic tests in the plant industry. The first section of this review focuses on rapid immunodiagnostic and DNA-based detection methods for known bacterial pathogens in plants or plant products, which often manifest no symptoms of disease. The second section covers the broader topic of disease diagnosis and new methods for identifying and characterizing bacteria.
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