Oxidative stress in hemodialysis--postdialytic changes.

Area of Science:

• Nephrology
• Biochemistry
• Oxidative Stress Research

Background:

• Hemodialysis can generate free oxygen radicals, causing oxidative stress.
• The duration of this oxidative stress and its persistence between dialysis sessions is unknown.
• Understanding oxidative stress carry-over is crucial for managing hemodialysis patients.

Purpose of the Study:

• To determine if oxidative stress from a hemodialysis session persists until the next session.
• To investigate the impact of different dialysis membranes and dialysates on oxidative stress carry-over.
• To assess the influence of membrane re-use on oxidative stress markers.

Main Methods:

• Evaluated four membrane/dialysate combinations: Polysulphone-Bicarbonate (PB), Polysulphone-Acetate (PA), Cuprophan-Acetate (CA), and Cuprophan-Bicarbonate (CB).
• Collected blood samples at multiple time points (pre-dialysis, 4, 6, 12, 24, 48 hours) across two consecutive dialysis sessions.
• Measured plasma and erythrocyte lipid peroxides, Vitamin E, Vitamin A, Superoxide Dismutase (SOD), and Glutathione Peroxidase (GP).

Main Results:

• Plasma lipid peroxide levels increased post-dialysis, while erythrocyte lipid peroxide levels decreased.
• Plasma Vitamin E increased significantly after initial use but not after re-use.
• No significant changes were observed in plasma Vitamin A or erythrocyte SOD/GP levels.
• Crucially, no parameters showed significant changes between pre-dialysis and 48/96-hour samples, indicating no carry-over.

Conclusions:

• Hemodialysis-induced oxidative stress does not carry over to the subsequent dialysis session.
• The type of dialysis membrane or dialysate used does not influence this lack of carry-over.
• These findings suggest that oxidative stress is transient and resolved before the next dialysis session.