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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
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Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

Seventeen-colour flow cytometry: unravelling the immune system

Stephen P Perfetto1, Pratip K Chattopadhyay, Mario Roederer

  • 1ImmunoTechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Disease, National Institutes of Health, 40 Convent Drive, Room 5507, Bethesda, Maryland 20892-3015, United States. sperfetto@nih.gov

Nature Reviews. Immunology
|August 3, 2004
PubMed
Abstract

No abstract available in PubMed .

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Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...