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A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
10:18

A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Published on: April 25, 2015

[Analysis of the resistance gene in Streptococcus pneumoniae]

Hitoshi Miyamoto1, Mitsuharu Murase

  • 1Department of Clinical Laboratory, Ehime University Hospital.

Kansenshogaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
|August 4, 2004
PubMed

Insights

Streptococcus pneumoniae strains show increasing resistance to antibiotics like penicillin and macrolides due to gene mutations. This rise in antimicrobial resistance, particularly in fluoroquinolone-resistant strains, signals future treatment challenges.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Context:

  • Investigated antimicrobial resistance in *Streptococcus pneumoniae*.
  • Analyzed 81 clinical isolates from Ehime University Hospital (2002-2003).
  • Focused on resistance mechanisms in penicillin-binding proteins (PBPs), macrolide resistance genes, and fluoroquinolone resistance genes.

Purpose:

  • Determine the prevalence of specific resistance genes in *Streptococcus pneumoniae*.
  • Identify mutations in penicillin-binding protein (PBP) genes (pbp2x, pbp2b, pbp1a).
  • Characterize macrolide resistance genes (mefA, ermB) and fluoroquinolone resistance genes (gyrA, parC).

Summary:

  • High rates of PBP mutations were observed, with combined mutations in pbp2x and pbp2b being common.
  • The *ermB* gene was the predominant macrolide resistance gene, found in 56.8% of isolates.
  • Fluoroquinolone resistance was associated with *gyrA* and *parC* gene mutations, with *parC* mutations being more frequent.

Impact:

  • Observed a concerning increase in multi-drug resistant strains, including those with PBP and *ermB* gene mutations.
  • Found a high co-occurrence of *gyrA*/*parC* mutations with PBP and macrolide resistance genes.
  • Suggests a growing trend of antimicrobial resistance in *Streptococcus pneumoniae*, necessitating further surveillance and treatment strategy development.

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