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Related Experiment Videos

Nuclear localization of the DOCK180/ELMO complex.

Jinhu Yin1, Lisa Haney, Scott Walk

  • 1Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MA 21224, USA.

Archives of Biochemistry and Biophysics
|August 4, 2004
PubMed
Summary

The DOCK180 protein forms a nuclear complex with ELMO proteins, exhibiting Rac-guanine nucleotide exchange factor (GEF) activity. This complex

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • DOCK180 protein is crucial for development, cell motility, and phagocytosis.
  • DOCK180 forms a complex with ELMO proteins, functioning as a Rac-guanine nucleotide exchange factor (GEF).
  • The precise nature, localization, and composition of DOCK180-containing complexes were previously unclear.

Purpose of the Study:

  • To biochemically characterize endogenous DOCK180-containing complexes.
  • To determine the subcellular localization and functional activity of DOCK180 complexes.
  • To investigate the association of DOCK180 with different ELMO isoforms.

Main Methods:

  • Biochemical purification of endogenous DOCK180 complexes.
  • Size exclusion chromatography to determine complex molecular weight.

Related Experiment Videos

  • Analysis of DOCK180 and ELMO isoform interactions in various cell lines.

    • Main Results:

    • A significant portion of endogenous DOCK180 exists as a 700kDa nuclear complex with ELMO proteins.
    • This nuclear DOCK180/ELMO complex demonstrates functional Rac-GEF activity.
    • DOCK180 associates with different ELMO isoforms (ELMO1, 2, or 3) depending on the cell type and expressed isoforms.

    Conclusions:

    • DOCK180 forms distinct, cell-type specific complexes with various ELMO proteins.
    • These DOCK180/ELMO complexes possess Rac-GEF activity and function in the nucleus.
    • DOCK180 may have dual roles in both nuclear and cytoplasmic cellular processes.