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Related Experiment Videos

Mouse antibody production test: can we do without it?

E Mahabir1, K Jacobsen, M Brielmeier

  • 1Department of Comparative Medicine, GSF--National Research Centre for Environment and Health, D-85764 Neuherberg, Germany. schmidt@gsf.de

Journal of Virological Methods
|August 4, 2004
PubMed
Summary
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Comparing diagnostic methods for mouse viruses like MHV-A59 and MMVp is crucial. The study found that the mouse antibody production test and PCR offer superior sensitivity for detecting these common murine pathogens.

Area of Science:

  • Veterinary Virology
  • Laboratory Animal Science
  • Diagnostic Microbiology

Background:

  • Microbiological contamination in experimental mice can lead to infections and compromise research integrity.
  • Sensitive, reliable, and routine diagnostic methods are essential for screening biological materials used in animal research.

Purpose of the Study:

  • To compare the sensitivity of three common diagnostic assays: viral plaque assay, mouse antibody production (MAP) test, and polymerase chain reaction (PCR).
  • To evaluate these methods for the detection of two prevalent murine viruses: Mouse Hepatitis Virus strain A59 (MHV-A59) and Murine Minute Virus (MMVp).

Main Methods:

  • Comparative analysis of viral plaque assay, MAP test (day 28), and reverse transcription PCR (RT-PCR) for MHV-A59 detection.
  • Comparative analysis of viral plaque assay, MAP test (day 28), and PCR for MMVp detection.

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  • Serial tenfold dilutions of virus stocks were used to determine assay sensitivity.

    • Main Results:

    • For MHV-A59, the MAP test (10^-10 dilution) was 10-fold more sensitive than the plaque assay (10^-9) and 10^4-fold more sensitive than RT-PCR (10^-6).
    • For MMVp, PCR (10^-10 dilution) was 10^6-fold more sensitive than both the plaque assay and MAP test (10^-4 dilution).
    • Assay sensitivity varied significantly depending on the specific virus being targeted.

    Conclusions:

    • The choice of diagnostic method for murine viruses should be based on determined sensitivity for the specific virus of interest.
    • The most sensitive detection method must be independently established for each virus to ensure accurate screening of biological materials.
    • Implementing sensitive diagnostic protocols is vital for maintaining the health of experimental mouse colonies and the validity of research outcomes.