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Updated: Aug 23, 2026

Cell-Free DNA Extraction of Vitreous and Aqueous Humor Specimens for Diagnosis and Monitoring of Vitreoretinal Lymphoma
Published on: January 12, 2024
Bilaterally identical monoclonality in a case of primary intraocular lymphoma
Tomomi Higashide1, Masayuki Takahira, Hirokazu Okumura
1Department of Ophthalmology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan. eyetomo@kenroku.kanazawa-u.ac.jp
Purpose:
To report a case of primary intraocular lymphoma possessing bilaterally identical monoclonal rearrangements of the immunoglobulin heavy chain (IgH) gene.
Design:
Observational case report.
Methods:
A 78-year-old woman with bilateral vitritis, subretinal infiltrates in the right eye, and no involvement of the central nervous system (CNS) was diagnosed histologically with diffuse large B-cell lymphoma by a transvitreal subretinal biopsy of the right eye. One month later, vitrectomy was performed on the left eye due to increased vitreous opacity. DNA was extracted from a vitrectomy specimen from each eye, and the third complementarity-determining region of IgH gene was analyzed by polymerase chain reaction and direct sequencing.
Results:
Monoclonal rearrangements of IgH gene with identical DNA sequence were detected in both eyes.
Conclusions:
Bilaterally identical monoclonality was detected in a case of primary intraocular lymphoma with no CNS involvement.
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