Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A non-EST-based method for exon-skipping prediction.

Rotem Sorek1, Ronen Shemesh, Yuval Cohen

  • 1Department of Human Genetics, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Israel. rotem@compugen.co.il

Genome Research
|August 4, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthetically designed anti-defense proteins overcome barriers to bacterial transformation and phage infection.

Nature communications·2026
Same author

Phage RyR-domain proteins degrade ADPR-based immune signals and fuel NAD<sup>+</sup> synthesis.

bioRxiv : the preprint server for biology·2026
Same author

Functional diversity of phage sponge proteins that sequester host immune signals.

Nature microbiology·2026
Same author

Bacterial defense via RES-mediated NAD<sup>+</sup> depletion is countered by phage phosphatases.

Cell host & microbe·2026
Same author

Histone H1 variants regulate neurodevelopmental transcriptional programs in autism with 16p11.2 deletion.

Genome biology·2026
Same author

Extracellular activity of a bacterial protease associated with reduced phage infectivity.

PloS one·2026
Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026
Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026
Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026
Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026
Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026
Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026
See all related articles

Scientists developed a novel genomic method to predict cassette exons, a type of alternative splicing. This approach bypasses the need for expressed sequence tags (ESTs) and detects previously undiscovered splice variants.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Alternative splicing is a key mechanism in gene expression, with 35-74% of human genes undergoing this process.
  • Current large-scale detection methods like expressed sequence tags (ESTs) and microarrays only sample the transcriptome, limiting the detection of rare splice variants.

Purpose of the Study:

  • To develop a novel method for predicting alternative splicing, specifically cassette exon skipping.
  • To identify splice variants not detectable by current EST-based methods.

Main Methods:

  • A new prediction method was developed based solely on the genomic sequence of internal exons and their mouse orthologs.
  • No external data, such as ESTs or cDNA, are required for the prediction.

Related Experiment Videos

Main Results:

  • The method was experimentally validated.
  • Hundreds of novel splice variants were detected that were previously undetectable using ESTs.
  • A significant portion of human genome splice variants remain unidentified by current EST or cDNA data.

Conclusions:

  • The novel genomic sequence-based method effectively predicts cassette exons.
  • This approach significantly expands the detection of alternative splicing variants beyond current limitations.
  • Many splice variants in the human genome are likely missed by existing transcriptome sampling techniques.