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Updated: Aug 23, 2026

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
Published on: May 10, 2018
(Pro)Insulin processing: a historical perspective
1Barbara Davis Center for Childhood Diabetes, University of Colorado Health Sciences Center, Denver, CO, USA. Howard.Davidson@UCHSC.edu
Abstract:
Insulin, the major secreted product of the beta-cells of the islets of Langerhans, is initially synthesized as a precursor (preproinsulin), from which the mature hormone is excised by a series of proteolytic cleavages. This review provides a personal narrative of some of the key research projects leading to the identification of the central processing enzymes as proprotein convertase 1, proprotein convertase 2, and carboxypeptidase E. It also discusses the central roles of the intragranular environment and chaperone-like proteins in modulating processing activity.
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