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D-hexosaminate production by oxidative fermentation.

D Moonmangmee1, O Adachi, H Toyama

  • 1Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok, 10140, Thailand. duangtip.moo@kmutt.ac.th

Applied Microbiology and Biotechnology
|August 4, 2004
PubMed
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Acetic acid bacteria efficiently produce D-hexosaminates through oxidative fermentation using membrane-bound D-glucosamine dehydrogenase. This method allows for stable accumulation of D-hexosaminates and was used to prepare novel compounds.

Area of Science:

  • Microbiology
  • Biotechnology
  • Biochemistry

Background:

  • Acetic acid bacteria possess membrane-bound enzymes capable of oxidizing D-hexosamines.
  • D-glucosamine dehydrogenase, distinct from quinoprotein D-glucose dehydrogenase, plays a key role in this oxidation.
  • Previous studies have not fully explored the microbial production of D-hexosaminates.

Purpose of the Study:

  • To investigate the microbial production of D-hexosaminates via oxidative fermentation using acetic acid bacteria.
  • To characterize the enzyme responsible for D-hexosamine oxidation and its localization.
  • To explore the potential of using whole cells (growing, resting, dried, immobilized) as catalysts for D-hexosaminate production.

Main Methods:

  • Oxidative fermentation of D-hexosamines using various strains of acetic acid bacteria, primarily Gluconobacter frateurii IFO 3264.

Related Experiment Videos

  • Enzymatic assays to confirm the activity and localization of D-glucosamine dehydrogenase.
  • Chromatographic separation techniques for isolating and purifying produced D-hexosaminates.

    • Main Results:

    • Most acetic acid bacteria strains efficiently converted D-hexosamines to corresponding D-hexosaminates stoichiometrically.
    • D-hexosaminates were stably accumulated in the culture medium, even after substrate depletion.
    • Resting, dried, and immobilized cells proved effective catalysts for D-hexosaminate production.
    • D-Mannosaminate and D-galactosaminate were synthesized for the first time using this method.

    Conclusions:

    • Acetic acid bacteria are effective biocatalysts for the microbial production of D-hexosaminates through oxidative fermentation.
    • The membrane-bound D-glucosamine dehydrogenase is crucial for this biotransformation.
    • Immobilized cell systems offer a practical approach for industrial-scale D-hexosaminate synthesis.