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Time course and inhibition of saponin-induced hemolysis
M Takechi1, S Shimada, Y Tanaka
1Faculty of Pharmaceutical Sciences, Kinki University, Higashiosaka, Japan.
Planta Medica
|April 1, 1992
Abstract:
Hemolytic activities of 3 steroid saponins reached plateaus within 5 min, whereas those of 4 triterpenoid saponins did not within 60 min. Erythrocytes pretreated with a low concentration of tigogenin were resistant to hemolysis of some of these saponins, but those pretreated with hecogenin or tomatidine were as sensitive as non-treated erythrocytes. Therefore, the ketone group of hecogenin or the amino group of tomatidine would weaken the interactions between the erythrocytes and these sapogenins. Furthermore, incubations of these saponins with a small amount of cholesterol diminished the hemolytic activities.