[The influence of abnormally higher WBC on hemoglobin determination and its redress]

To discuss the influence of abnormally higher white blood cell (WBC) on hemoglobin (Hb) determination and work for its redress.

High WBC concentration suspensions of 48 concentration gradients were prepared to be determined in the pre-dilution pattern of automated hematology analyzer while the changes of Hb concentration before and after removal of WBC being observed. At the same time, a regression equation was set up to correct errors and redress the Hb concentration. Then, the data from 14 whole blood samples with abnormally higher WBC were used in this regression equation to verify its utility.

The Hb concentration of samples with abnormally higher WBC was higher than that of samples where WBC was removed (P<0.001). While WBC count > or = 40.0 x 10(9)/L, there was logarithmic linear correlation between Hb concentration difference and WBC count (r=0.9526). Using the regression equation Y (Hb difference) = -25.09 + 21.89 x lg[WBC (x 10(9)/L)], we found that there was no significant difference (P>0.05) between the "practically detected Hb difference" and the "theoretically adjusted Hb difference" in the whole blood samples with abnormally higher WBC.

Abnormally higher WBC may lead to increase of error in the detection of Hb concentration; the ultimate result in close proximity to true Hb concentration of whole blood samples with abnormally higher WBC can be acquired through the linear regression equation.