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Updated: Aug 23, 2026
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Published on: November 23, 2012
[Purification and characterization of sanguicin--a bacteriocin produced by Streptococcus sanguis]
Hui Deng1, Yi Ding, Ming-de Fu
1Department of Oral Medicine, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Objective:
Streptococcus sanguis plays an important Role in maintaining the periodontal microecological balance. Previous studies have demonstrated that sanguicin--a kind of bacteriocin produced by S. sanguis has a prominent function of inhibiting the growth of putative periodontopathic bacteria (PPB). The aim of this study was to purify sanguicin and investigate its characters.
Methods:
The raw extract of sanguicin was obtained from cells of S. sanguis by ultrasonication, salting out and dialysis. Then the diethyl-aminoethyl-sepharose gradual washing was done in conjunction with glucosan gel filtration for in the purfication of sanguicin. The characters of purified sanguicin were determined and its inhibitiory effect on PPB was measured by agar diffusion test.
Results:
The purifed sanguicin was obtained; it was heat labile and proteinaceous; and by SDS-PAGE, it showed a main band with the relative molecular mass of 65 x 10(3). The purifed sanguicin had a strong inhibitory effect on P. gingivalis, P. intermedia and F. nucleatum in vitro.
Conclusion:
Our methods are useful for the purification of sanguicin. Sanguicin has inhibitory effect on PPB obviously and will have a good prospect in periodontal ecological therapy.
