Specific band observed in VCD predicts the anomeric configuration of carbohydrates
Kenji Monde1, Tohru Taniguchi, Nobuaki Miura
1Division of Biological Sciences, Graduate School of Science, Frontier Research Center for Post-Genomic Science and Technology, Hokkaido University, Kita-ku, Sapporo 001-0021, Japan. kmonde@glyco.sci.hokudai.ac.jp
Abstract:
The vibrational circular dichroism (VCD) technique was applied to carbohydrates and produced a glycoside characteristic VCD band, which was named the "glycoside band." It confirmed that this vibration was related to anomeric configurations of carbohydrates in the case of the d-series by means of isotope substitution. This band is defined as a negative, sharp, and intense VCD band at around 1145 cm-1, exhibited by d-sugars with an axial alpha-glycosidic linkage in the 4C1 conformation. As applications of this band, the VCD studies of the maltose-cellobiose mixture and the monitoring of the amyloglucosidase reaction were examined.
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