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Related Experiment Videos

Microarray analysis of gene expression after transverse aortic constriction in mice.

Mingming Zhao1, Amy Chow, Jennifer Powers

  • 1Department of Pediatrics, Stanford University, Stanford, California 94304, USA.

Physiological Genomics
|August 5, 2004
PubMed
Summary

Cardiac hypertrophy involves complex gene expression changes. Microarray analysis in a mouse model revealed significant alterations in gene pathways regulating cardiac structure and function over time.

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Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Genomics

Background:

  • Cardiac hypertrophy is an adaptive response that can progress to heart failure.
  • Understanding the molecular mechanisms of cardiac hypertrophy is crucial for developing therapeutic strategies.
  • Gene expression profiling offers a powerful approach to uncover these mechanisms.

Purpose of the Study:

  • To identify novel adaptive mechanisms in cardiac hypertrophy using gene expression profiling.
  • To characterize the temporal changes in gene expression during compensated pressure overload hypertrophy.
  • To validate findings using quantitative real-time RT-PCR.

Main Methods:

  • Utilized a murine model of compensated pressure overload hypertrophy induced by transverse aortic constriction (TAC).

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  • Employed Affymetrix U74Av2 GeneChips for microarray analysis of cardiac gene expression at 48 hours, 10 days, and 3 weeks post-TAC.
  • Performed SYBR quantitative real-time RT-PCR (QRT-PCR) for validation of selected gene expression changes.

    • Main Results:

    • Identified significant upregulation and downregulation of numerous genes at different time points after TAC.
    • Observed alterations in transcripts related to cell proliferation (e.g., Ki-67) and apoptosis.
    • Demonstrated strong correlation between microarray data and QRT-PCR validation, particularly for highly differentially expressed genes.

    Conclusions:

    • Transverse aortic constriction induces widespread alterations in cardiac gene expression, impacting pathways regulating cardiac structure and function.
    • Microarray analysis is a valuable tool for dissecting the complex molecular landscape of cardiac hypertrophy.
    • Rigorous controls, statistical analysis, and validation are essential for reliable in vivo gene expression studies.