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Updated: Aug 23, 2026
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
[Expression of proliferating cell nuclear antigen/bcl-2 in gray gingival tissue]
Lin Chen1, Guo-wei Zhu, Xiao-hong Yang
1Dept. of Prosthodontics, Stomatological Hospital of Zunyi Medical College, Zunyi 563003, China.
Objective:
To study the expression of PCNA/bcl-2 protein in gray and normal gingival tissue, and to investigate the biological effect of procelain-fused-to-metals (PFM).
Methods:
Gray gingival tissue animal model was established by PFM prosthesis and immunohistochemistry S-P method was used to detect the PCNA/bcl-2 protein expression in gingival tissue after 3 months, 6 months in PFM groups and control group.
Results:
The expression of PCNA had significant difference between the 3th month group and the control group (P < 0.05); no significant difference was found between the 6th month group and the control group (P > 0.05). The expression of bcl-2 had no significant difference between the 6th month group and the control group (P > 0.05).
Conclusion:
There were a correlation between the expression of PCNA and inflammation of the gray gingival tissue; The expression of PCNA and bcl-2 protein does not indicate that gray gingival tissue had dysplasia change.
