Related Experiment Video
Updated: Aug 23, 2026
Mouse Embryonic Development in a Serum-free Whole Embryo Culture System
Published on: March 1, 2014
[Establishment of a culture system of chick embryo for mouse tooth germ development]
Zheng-bin Yan1, Wei-dong Tian, Lei Liu
1Key. Laboratory of Oral Biomedical Engineering Ministry of Education, Sichuan University, Chengdu 610041, China.
Objective:
To establish a new culture system for mouse tooth germs in chick embryo.
Methods:
The mandibular first molar germ fragments of 15 embryonic days' Kunming mouse embryo were implanted into the lateral mesenchyme of 4-5 days' chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.
Results:
The cultured tooth germ development continued from cap stage to latest bell stage. The ameloblast and the odontoblast all differentiated maturely and secreted matrix.
Conclusion:
4-5 days' wing buds chick embryo could serve as developing the mouse tooth germs and demonstrate well physiological process of differentiation and morphogenesis.
