An improved 'cold SSCP' method for the genotypic and subgenotypic characterization of Cryptosporidium
Robin B Gasser1, Youssef G Abs EL-Osta, Sandra Prepens
1Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Vic. 3030, Australia. robinbg@unimelb.edu.au
Molecular and Cellular Probes
|August 6, 2004
Abstract:
A simple, non-isotopic PCR-based single-strand conformation polymorphism ('cold SSCP') method is described which allows the efficient detection of genetic variation among and within genotypes of Cryptosporidium parvum. This low cost approach has important advantages over other 'genotyping' methods and is applicable to a wide range of genetic loci and organisms.
