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Updated: Aug 23, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
GMPOZ, a BTB/POZ domain nuclear protein, is a regulator of hormone responsive gene expression in barley aleurone
Fiona J Woodger1, John V Jacobsen, Frank Gubler
1CSIRO Plant Industry, GPO Box 1600, Canberra, ACT, 2601, Australia. woodger@rsbs.anu.edu.au
Abstract:
GAMYB is a GA-responsive activator of hydrolase gene expression in the aleurone layer of germinated cereal grains. We have isolated a putative GAMYB-binding protein, GMPOZ, which contains a BTB/POZ domain found in certain animal transcriptional regulators. Although BTB/POZ domain proteins are numerous in plants, very few are yet characterized. We found that GMPOZ is nuclear localized and that GMPOZ mRNA is expressed highly in anthers as well as aleurone. Transient silencing of the GMPOZ gene suggests that GMPOZ is involved in hormone responsive gene expression in aleurone.
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